Parameters of [3H]DAMGO binding in membranes
| Treatment | [3H]DAMGO binding | ||
|---|---|---|---|
| Kd | Bmax | Reduction in Bmax | |
| nm | fmol/mg | % | |
| Control | 0.48 ± 0.11 | 8219 ± 582 | |
| DAMGO | 0.39 ± 0.046 | 1465 ± 280 | 82 |
| Morphine | 1.29 ± 0.24 | 3373 ± 423 | 59 |
| Butorphanol | 2.17 ± 0.57 | 4140 ± 434 | 50 |
| Etorphine | 3.63 ± 0.51 | 244 ± 59 | 97 |
| β-Endorphin | 0.41 ± 0.06 | 1212 ± 60 | 85 |
Equilibrium binding of [3H]DAMGO was carried out in membranes prepared from control and agonist-treated cells (10 μm for 24 hr) as described in Experimental Procedures.
Values are mean ± standard error from three or four experiments.