Primers used for amplification of α1-AR subtype transcripts by RT-PCR
| Primer | Sequence | Position | Annealing temperature |
|---|---|---|---|
| ° | |||
| α1A-AR forward | 5′-GTAGCCAAGAGAGAAAGCCG-3′ | 628–647 | 50 |
| α1A-AR reverse | 5′-CAACCCACCACGATGCCCAG-3′ | 820–839 | 50 |
| α1B-AR forward | 5′-GCTCCTTCTACATCCCGCTCG-3′ | 629–649 | 58 |
| α1B-AR reverse | 5′-AGGGGAGCCAACATAAGATGA-3′ | 908–928 | 58 |
| α1D-AR forward | 5′-CGTGTGCTCCTTCTACCTACC-3′ | 759–779 | 58 |
| α1D-AR reverse | 5′-GCACAGGACGAAGACACCCAC-3′ | 1042–1062 | 58 |
The sequences of the primers (listed from 5′ to 3′) used for RT-PCR analysis are shown. The optimized annealing temperature for the amplification of mouse transcripts by each primer pair is also shown. The primer sequences were determined from rat in a previous report (22).