Amino acid substitutions | CYP2D6 holo-protein level1-a | Reductase level |
---|---|---|
pmol/mg | ||
None | 11.6 ± 5.4 | 1660 ± 350 |
T107I | 10.8 ± 6.8 | 1520 ± 500 |
R296C | 9.7 ± 4.1 | 1490 ± 540 |
S486T | 11.0 ± 3.9 | 1710 ± 470 |
T107I + R296C | 8.0 ± 2.7 | 1530 ± 300 |
T107I + S486T | 8.8 ± 3.4 | 1520 ± 490 |
R296C + S486T | 7.2 ± 2.3 | 1640 ± 480 |
T107I + R296C + S486T | 6.1 ± 3.6 | 1530 ± 320 |
↵1-a The endogenous P450 levels obtained in microsomes from yeast transfected with the empty V60 vector (0.6 ± 0.6 pmol/mg) have been subtracted from the levels obtained in the microsomes from CYP2D6 expressing yeast cells.