Table 4

Basal levels of cAMP in nontransfected cells or cells expressing D1, D2, and chimeric dopamine receptors

Assay temperaturecAMP
HEK 293 cellsD1D2[1-4,7]D2[1-5,7]D2
pmol/well
37°4.3  ± 1.128.6  ± 3.24-a 101.5  ± 8.84-a 6.4  ± 1.04-b , 4-c 6.3  ± 1.04-b , 4-c
40°34.3  ± 6.2100  ± 6.24-c

Basal cAMP levels were determined as described in Experimental Procedures. Results shown are mean from ≥10 independent experiments ± standard error.

    • ↵4-a Significantly different from nontransfected HEK 293 cells, p < 0.01.

    • ↵4-b Significantly different from D2[1-4,7], P < 0.01.

    • ↵4-c Significantly different from D1,P ≳ 0.02.