Table 2

Inhibitory effects of control compounds DS, AZT, Ro5-3335, and Saquinavir and AdoHcy hydrolase inhibitors on HIV-1 replication

	Inhibition of virus-induced LacZ expression, IC₅₀^2-a	Toxicity, CC₅₀^2-b
	μm
DS 5000	0.007 ± 0.001	>2
AZT	0.00034 ± 0.000022	>0.04
Ro5-3335	0.1 ± 0.05	3.36 ± 2.58
Saquinavir	0.8 ± 0.5	>15
NPA	1.9 ± 2.7	30 ± 31
c³NPA	0.41 ± 1.5	76 ± 21
DHCaA	1.3 ± 1	55
c³DHCaA	0.29 ± 0.0042	37 ± 25
C-c³Ado	7.4 ± 5	>37
6′-(R)-Methyl-NPA	3.5 ± 0.5	>36
6′-(S)-Methyl-NPA	>36	>36
(S)-DHPA	248 ± 189	>191
c⁷DHCaA	51	>42
6′-(R)-Ethynyl-NPA	38	>69
6′-(S)-Ethynyl-NPA	>104	>104
6′-(R)-Ethenyl-NPA	6.16 ± 3	>69
6′-(S)-Ethenyl-NPA	57 ± 52	>138
6′-(R)-Ethyl-NPA	4.6 ± 0.34	>68
6′-(S)-Ethyl-NPA	67 ± 3.7	>68

↵2-a Concentration of the inhibitor required for 50% inhibition of β-Gal expression driven by the HIV-1 LTR in HIV-1-infected CD4⁺ HeLa cells.
↵2-b Concentration of the inhibitor required for 50% inhibition of total protein expression in cell culture.
Data represent mean values of two to four separate experiments, each performed in triplicate.