Effect of natural nucleosides on the antiviral effects of S2242, ACV, and DHPG
| Nucleoside added | EC50 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| HCMV (Davis) | HSV-1 (KOS) | VZV5-a | HHV-6 (GS) | ||||||
| DHPG | S2242 | DHPG | ACV | S2242 | DHPG | ACV | S2242 | S2242 | |
| μg/ml | |||||||||
| None | 0.7 | 0.02 | 0.008 | 0.5 | 0.2 | 0.5 | 0.1 | 0.03 | 0.005 d |
| 2’-Deoxythymidine | 2.5 | 0.04 | 12 | >200 | 0.9 | 17.5 | 7.6 | 0.8 | |
| Cytidine | 0.008 | 0.2 | 0.9 | 2.2 | 1.0 | 0.6 | |||
| 2’-Deoxycytidine | 1.5 | 0.1 | 0.24 | 4.5 | 18 | 12 | 11 | 1.8 | 0.3 d |
| Adenosine | 3.5 | 0.03 | 0.008 | 2.5 | 0.2 | 0.94 | 1.9 | 0.04 | |
| 2’-Deoxyadenosine | 0.5 | 0.04 | 0.008 | 0.2 | 0.6 | 3.0 | 1.1 | 0.09 | |
| Guanosine | 3.0 | 0.03 | 0.02 | 0.9 | 0.2 | 0.82 | 0.25 | 0.02 | |
| 2’-Deoxyguanosine | 5.0 | 0.01 | 0.11 | 15 | 0.55 | 11.9 | 1.2 | 0.04 | |
All nucleosides were added at a final concentration of 100 μg/ml, except for guanosine, which was added at 25 μg/ml in the HCMV and HSV assays. Data are mean values for at least two or three separate experiments.
↵5-a Mean values for strains Oka and YS.
5-b Similar values were obtained when the cultures were analyzed for HHV-6 antigen expression by FACS analysis (i.e., EC50 value for the control, 0.003 μg/ml, compared to 0.25 μg/ml in the presence of dCyd).