Form transfected | PDE activity |
---|---|
pmol/min/mg of protein | |
Mock-transfected cells | 23.2 ± 1.2 |
Rat PDE4A | 529.01 ± 249 |
Rat PDE4B | 719.62 ± 40 |
Rat PDE4D | 1549.0 ± 572 |
MA-10 cells were transfected with 10–15 μg of plasmid according to the CaPO4 method. Mock-transfected cells received no plasmid. Experiments done using the vector only gave results identical to those with mock transfection. Cells were harvested 24 hr after transfection, and PDE activity in the homogenate was measured. Each value is the mean ± standard error of at least three transfections performed on different days.