Efflux parameters of anthracycline derivatives by GLC4/ADR cells
| Anthracycline | ka × 1010 | VM | Km | nH |
|---|---|---|---|---|
| s−1 (cells/ml)−1) | × 10−5molecules/cell/sec | μm | ||
| eso-DNR | 17 | 16.2 ± 1.2 | 0.8 ± 0.1 | 1.9 ± 0.5 |
| DNR | 13 | 12.0 ± 1.2 | 0.8 ± 0.2 | 1.8 ± 0.5 |
| PIRA | 14 | 15.0 ± 1.8 | 0.9 ± 0.2 | 1.8 ± 0.4 |
| Br-DNR | 15 | 7.2 ± 0.6 | 0.42 ± 0.03 | 2.6 ± 0.4 |
| OH-DNR | 2 | 1.6 ± 0.2 | 0.6 ± 0.1 | 1.9 ± 0.8 |
| OH-DOX | 2 | 2.4 ± 1.2 | 1.3 ± 1.1 | 1.3 ± 0.7 |
Data are mean ± standard deviation from three to five independent experiments on different days. ka was calculated using eq. 5, where VM was expressed in m/sec/(cells/ml), the conversion being 1m/sec/(cells/ml) = 6 × 1014molecules/cell/sec.