Kinetic parameters of EAAT1 and EAAT2
| Ki/Kb | ||
|---|---|---|
| EAAT1 | EAAT2 | |
| μm | ||
| Uptake assay | ||
| dl-TBOA | 42 ± 19 | 5.7 ± 1.1 |
| dl-TBzOAsp | 149 ± 33 | 83 ± 7.7 |
| dl-THA | 58 ± 24 | 14 ± 4.6 |
| DHKA | >1000 | 79 ± 19 |
| l-CCG-III | 7.5 ± 3.8 | 2.5 ± 0.4 |
| Electrophysiology | ||
| dl-TBOA | 9.0 | 0.116 |
Inhibition constants (K i) were obtained from initial rates of glutamate uptake measured at various concentrations of glutamate and inhibitors (Fig. 3). Antagonist equilibrium dissociation constants (K b) were obtained from Schild analysis (Fig. 5).