GABA | Diazepam potentiation | ||||
---|---|---|---|---|---|
EC50 μM | nH | Maximum potentiation | EC50 | nH | |
μM | nm | ||||
α1β2γ2 | 12.7 ± 0.7 | 1.07 ± 0.05 | 1.91 ± 0.17 | 51 ± 6 | 1.11 ± 0.10 |
α1β2 | 8.2 ± 0.4 | 0.93 ± 0.04 | |||
β2γ2 | 210 ± 12 | 0.75 ± 0.03 | 1.03 ± 0.07 | 24 ± 2 | 0.89 ± 0.06 |
α1β2χ161 | 17.4 ± 1.4 | 0.93 ± 0.06 | 0.26 ± 0.01 | 295 ± 34 | 1.41 ± 0.12 |
α1β2χ167 | 14.9 ± 1.3 | 0.96 ± 0.06 | 0.28 ± 0.02 | 324 ± 53 | 1.30 ± 0.17 |
Dose-response data for wild-type and chimeric subunit combinations for GABA and diazepam potentiation of GABA-mediated CI− current inX. laevis oocytes are tabulated. Two-electrode voltage-clamp and data analysis was performed as described (see Materials and Methods). Mean and standard deviation values for maximum potentiation, EC50 values, and Hill coefficients (n H) were calculated from dose-response data (Figs. 4b and 5) with the use of Prism software.