Condition | Adenosine incorporated into RNA | Thymidine incorporated into DNA | [3H]Thymidine nucleotides formed during the 1-hr pulse |
---|---|---|---|
pmol/10 6 cells/hr | fmol/10 6 cells/hr | fmol/10 6 cells | |
Control | 460 ± 149 | 547 ± 182 | 78 ± 17 |
IFN-α + γ hr −048 | 622 ± 60 | 277 ± 109 | 85 ± 18 |
(135%) | (50.6%) | (109%) | |
FUra hr 24 to 48 | 649 ± 54 | 377 ± 97 | 55 ± 17 |
(141%) | (68.9%) | (71%) | |
IFN-α + γ plus FUra | 651 ± 50 | 98 ± 36 | 63 ± 19 |
(142%) | (17.9%) | (81%) |
HT29 cells were treated with diluent, IFN-α + γ (500 units/ml and 10 units/ml), FUra (1 μm), or the combination as indicated. The cells then were incubated with [3H]adenosine (1 μCi) for 30–60 min or [3H]thymidine (1–2 μCi) for 1 hr. Incorporation into acid-precipitable material was determined. The data have been corrected for the total ATP and dTTP pool sizes (both endogenous and [3H]triphosphate pools). The data for RNA synthesis are presented as mean ± standard deviation (n = 3). [3H]Thymidine nucleotide formation (dTMP, dTDP, and dTTP) is shown as the mean ± standard error (n = 4). The data for DNA synthesis are presented as mean ± standard error (n = 6). The percentage of control values are shown in parentheses. The differences in the median values among the treatment groups for RNA synthesis and [3H]thymidine nucleotide formation are not significant (p = 0.313 and p = 0.375, respectively; Kruskal-Wallis one-way analysis of variance on ranks). However, the differences in the median values for DNA synthesis among the treatment groups are significant (p = 0.018), and pairwise multiple comparisons indicated that control and the three-drug combination were significantly different (p < 0.05)