Treatment | Immunoreactivity (relative to control) | |
---|---|---|
GLT-1 | GLAST | |
Control | 1.0 | 1.0 |
dbcAMP (0.25 mm) | 8.2 ± 2.9 | 2.3 ± 0.5 |
Forskolin (10 μm) | 2.2 ± 0.2 | 1.4 ± 0.2 |
8-Bromo-cAMP (0.25 mm) | 7.0 ± 0.7 | 1.7 ± 0.1 |
Isoproterenol (2 μm) | 0.84 ± 0.24 | 0.92 ± 0.44 |
Astrocytes were prepared as described in the text and maintained in medium supplemented with drugs starting at day 14. After 10 days of treatment, the levels of GLT-1 or GLAST immunoreactivity were determined by immunoblotting. Values represent the mean ± standard error of at least three independent observations.