Effects of PKA antagonists, receptor antagonists, and TTX on expression of GLT-1 and GLAST in cocultures of neurons and astrocytes
| Treatment | Immunoreactivity (relative to control) | |
|---|---|---|
| GLT-1 | GLAST | |
| Control | 1.0 | 1.0 |
| KT5720 (5 μm) | 0.83 ± 0.03 | 1.35 ± 0.22 (3) |
| H89 (2 μm) | 0.71 ± 0.12 | 0.93 ± 0.18 (5) |
| rpcAMP (0.5 mm) | 1.02 ± 0.08 | 0.84 ± 0.01 (2) |
| Propranolol (10 μm) | 0.79 ± 0.16 | 0.79 ± 0.21 (5) |
| (+)-MCPG (0.2 mm) | 0.92 ± 0.22 | 1.12 ± 0.11 (6) |
| MK801 (1 μm) | 0.83 ± 0.16 | 0.80 ± 0.07 (6) |
| TTX (1 μm) | 0.92 ± 0.19 | 0.86 ± 0.19 (5) |
Cocultures of neurons and astrocytes were prepared as described in the text. After 7 days, compounds were added the the medium to provide the final concentration indicated in parentheses. After an additional 7 days, cells were harvested, and the levels of GLT-1 and GLAST immunoreactivity were determined by immunoblotting. Values represent the mean ± standard error. The number of observations is provided in parentheses. There was no significant effect for any of the treatments as determined by analysis of variance.