Table 1

Effect of SNP on cGMP content in human prostatic SMCs.

cGMP accumulation
fmol/5 × 104 cells
Control4.3  ± 1.4
SNP
 0.1 μm 28.1  ± 10.2
 0.3 μm 43.8  ± 12.0
 1 μm 57.4  ± 10.3
 3 μm 58.1  ± 12.4

Cells were seeded onto wells (5 × 104 cells/p well). After a 24 hr incubation, cells were made quiescent for 48 hr by deprivation of serum. SNP was added to the sychronized cells for 5 min; then, cGMP was extracted and quantified by enzyme immunoassay.

    • Values represent mean ± standard error of four determinations.