cGMP accumulation | |
---|---|
fmol/5 × 104 cells | |
Control | 4.3 ± 1.4 |
SNP | |
0.1 μm | 28.1 ± 10.2 |
0.3 μm | 43.8 ± 12.0 |
1 μm | 57.4 ± 10.3 |
3 μm | 58.1 ± 12.4 |
Cells were seeded onto wells (5 × 104 cells/p well). After a 24 hr incubation, cells were made quiescent for 48 hr by deprivation of serum. SNP was added to the sychronized cells for 5 min; then, cGMP was extracted and quantified by enzyme immunoassay.
Values represent mean ± standard error of four determinations.