Inhibition of NO synthesis in RMC by Genistein and KT 5720
| Genistein | KT5720 | |
|---|---|---|
| Interleukin-1 | 29.4 μm | >5 μm |
| CGP 28238 | 2.5 μm | 0.82 μm 3-a |
| Forskolin | n.d. | 1.25 μm 3-a |
IC50-values for genistein and KT5720 on NO production in RMC were obtained by coincubating various concentrations of the inhibitors with the NOS inducers IL-1 (1 nm), CGP 28238 (100 μm), or the adenylate cyclase activator forskolin (5 μm) for 20 hr.
↵3-a No 100% inhibition was obtained within the concentration range of 10 nm to 3 μm.
n.d., not done.