in the presence of Forskolin | without Forskolin | |
---|---|---|
Control | 3.69 ± 0.17 | 0.15 ± 0.10 |
Rolipram (5 μm) | 11.3 ± 0.65 | 0.92 ± 0.74 |
CGP 28238 (100 μm) | 7.62 ± 0.95 | 0.59 ± 0.32 |
L-745337 (100 μm) | 3.44 ± 0.81 | 0.20 ± 0.10 |
NS-398 (100 μm) | 3.52 ± 0.42 | 0.23 ± 0.14 |
RMC were preincubated for 30 min with different methylsulfonanilides or rolipram after stimulation with forskolin (5 μm) or vehicle. Cyclic AMP levels (pmol/3 × 106) were measured 30 min after adenylate cyclase stimulation as described in Methods. The table describes the results of two different experiments. From two dose-response curves in the presence of forskolin, the EC50 of CGP 28238 in elevation of intracellular cAMP in RMC was calculated to 3.8 μm.