Hb | Bmax (moles of drug/moles of hemoglobin) | Kd |
---|---|---|
μM | ||
Hb A | ||
Sample 1 | 0.55 | 236 |
Sample 2 | 0.77 | 208 |
Sample 3 | 0.90 | 229 |
0.74 ± 0.182-a | 224 ± 152-a | |
Hb H | ||
Sample 1 | 1.86 | 74 |
Sample 2 | 1.59 | 46 |
Sample 3 | 1.56 | 77 |
1.67 ± 0.172-a | 66 ± 172-a |
The maximum drug bound per Hb molecule (B max) andK d of Hb binding to DHART were assayed by equilibrium dialysis. The values were calculated from binding curve (shown in Fig. 4) using the ENZFITTER program.
↵2-a Mean ± standard deviation.