Table 1

Rat brain cannabinoid receptor peptide fragments and corresponding antisera

Peptide	Amino acid sequence	Location	Antisera
CB1–14	MKSILDGLADTTFR(C)	Amino terminus	Anti-CB1–14
CB174–188	(K)SFVDFHVFHRKDSPN	EL1	Anti-CB174–188
CB301–317	KAHSHAVRMIQRGTQKS	N-side IL3	Anti-CB301–317
CB316–327	KSIIIHTSEDGK	Central IL3
CB329–344	QVTRPDQARMDIRLAK	C-side IL3
CB401–417	(K)RSKDLRHAFRSMFPSSE	Carboxyl terminus	Anti-CB401–417

The peptide numbers correspond to inclusive amino acid residues of each sequence of the CB₁ cannabinoid receptor as reported byMatsuda et al. (1990). Amino acids in parentheses were added to peptide sequences to facilitate coupling peptides to protein carriers. For peptide CB401–417, the amino-terminal lysine added for coupling was not present in the peptide used for biochemical studies. The underlined amino acid replaced a cysteine in the sequence.