Antisera dilution | Adenylate cyclase inhibition | Adenylate cyclase inhibition normalized to control | |
---|---|---|---|
% | |||
Experiment A | |||
None | 32.2 ± 4.1 | 100.0 | |
Anti-CB1–14 (n = 2) | 1:100 | 29.0 ± 7.7 | 90.1 |
1:20 | 29.7 ± 4.5 | 92.3 | |
1:5 | 19.4 ± 5.6 | 60.3 | |
Preimmune CB1–14 | 1:100 | 30.3 ± 4.0 | 94.1 |
1:20 | 28.6 ± 6.9 | 88.8 | |
1:5 | 16.9 ± 5.0 | 52.5 | |
Anti-CB174–188 (n = 2) | 1:100 | 33.7 ± 2.6 | 104.6 |
1:20 | 27.7 ± 9.6 | 86.0 | |
1:5 | 7.5 ± 4.9 | 23.3 | |
Preimmune CB174–188 | 1:100 | 35.9 ± 5.4 | 111.5 |
1:20 | 25.0 ± 3.4 | 77.6 | |
1:5 | 6.1 ± 2.3 | 18.9 | |
Experiment B | |||
None | 33.2 ± 1.2 | 100.0 | |
Anti-CB301–317 (n = 4) | 1:100 | 32.1 ± 5.2 | 96.7 |
1:20 | 14.3 ± 10.7 | 43.1 | |
1:5 | 7.1 ± 3.73-a | 21.2 | |
Preimmune CB301–317 | 1:100 | 32.9 ± 5.9 | 99.1 |
1:20 | 23.7 ± 3.1 | 71.4 | |
1:5 | 21.1 ± 9.3 | 63.5 | |
Anti-CB401–417 (n = 3) | 1:100 | 34.8 ± 1.4 | 107.4 |
1:20 | 29.4 ± 1.5 | 90.7 | |
1:5 | 10.8 ± 4.5 | 33.3 | |
Preimmune CB401–417 | 1:100 | 32.4 ± 2.6 | 100.6 |
1:20 | 26.9 ± 2.1 | 83.0 | |
1:5 | 12.2 ± 4.5 | 37.6 |
N18TG2 cell membrane preparations were preincubated with diluted preimmune sera or antisera at 4° for 4 hr before the addition to the adenylate cyclase assay mixture. Data were normalized for each experiment so the inhibition by 100 nm DALN in the absence of either antiserum or preimmune serum was 100% inhibition. Data are expressed as the mean ± standard error of two to four experiments, each with three determinations.
↵3-a Significantly lower than preimmune serum control atp < 0.05 using paired Student’s one-tailedt test.