Oxime | kr | ||
---|---|---|---|
Control | Edrophonium (50 μm) | Decamethonium (200 μm) | |
m −1·min−1 | |||
2-PAM1-a | |||
Fast | 273 ± 79 | 894 ± 115 | 434 ± 34 |
Slow | 33 ± 7 | 118 ± 41 | 74 ± 26 |
TMB4 1-b | |||
Fast | 1176 ± 77 | 6182 ± 479 | 2281 ± 397 |
Slow | 37 ± 7 | 325 ± 18 | 88 ± 15 |
LüH6 1-b | |||
Fast | 1114 ± 157 | 5003 ± 443 | 2117 ± 197 |
Slow | 30 ± 6 | 358 ± 31 | 89 ± 18 |
HI-61-a | |||
Fast | 736 ± 80 | 790 ± 196 | 604 ± 142 |
Slow | 54 ± 10 | 66 ± 24 | 52 ± 23 |
In all the cases, data were analyzed assuming a 50:50 ratio between the two kinetic species of EMP-AChE; for the determination ofk r, five or six concentrations of oxime were used ranging from 10 μm to 3 mm. Values are mean ± standard error of three or four determinations.
↵1-a Obtained by dividing k maxby K ox. In the case of 2-PAM, the changes of k r in the presence of the two ligands were mainly contributed by the decrease of K ox with no change in k max, whereas for HI-6, no such changes was detectable in the presence of either ligand.
↵1-b Calculated from the slope of k obs versus [oxime] plot.