Lipid Peroxidation in Hep G2 Subclones
| Cells | Addition | LPO |
|---|---|---|
| nmol of 4-HNE + MDA/mg of protein | ||
| C34 | 0 | |
| C34 | BSO | 0 |
| E47 | 0.03 ± 0.01* | |
| E47 | BSO | 0.16 ± 0.03* |
| E47 | VitE | 0 |
| E47 | VitE + BSO | 0.01 ± 0.01 |
C34 and E47 cells were cultured in MEM with or without 0.1 mM BSO and/or 20 μm VitE for 2 days. The lipid peroxidation levels in C34 and E47 cells were monitored by the production of lipid peroxidation end products, MDA and 4-HNE, as described in Materials and Methods. *, Compared to C34 cells (±BSO), p < 0.001.