Stopped-flow light scattering
| Drug | [Tubulin] (initial) | A15-a | τ |
|---|---|---|---|
| μm | sec | ||
| Vinflunine | 1.2 | 0.657 ± 0.085 | 20.90 ± 16.54 |
| 1.4 | 0.412 ± 0.094 | 7.82 ± 4.40 | |
| 1.8 | 0.964 ± 0.184 | 2.65 ± 1.43 | |
| 2.8 | 1.399 ± 0.186 | 2.14 ± 0.97 | |
| 4.0 | 1.080 ± 0.233 | 2.71 ± 0.80 | |
| Vinorelbine | 0.6 | 0.150 ± 0.018 | 25.25 ± 20.88 |
| 1.0 | 0.378 ± 0.013 | 15.71 ± 4.12 | |
| 2.0 | 1.738 ± 0.216 | 11.00 ± 2.02 | |
| 4.0 | 2.676 ± 0.189 | 10.68 ± 3.21 |
↵5-a A1 is the change in light scattering signal, and τ is the relaxation time.
Drug dilution from 70 to 35 μm, 10 mm PIPES, pH 6.9, 2 mm EGTA, 1 mm MgSO4, 50 μm GTP.