[3H]NMS binding: | ||
---|---|---|
L929-m3 | SH-SY5Y | |
fmol/mg protein | ||
Buffer/control | 680 | 456 |
Buffer/Oxo-M | 415 | 208 |
Sequestration | (39%) | (54%) |
WT/control | 751 | 409 |
WT/Oxo-M | 600 | 328 |
Sequestration | (20%) | (20%) |
Cells were pretreated with either vehicle alone (0.1% DMSO) or 10 μm WT for 15 min at 37° before the addition of either buffer or 1 mm Oxo-M for an additional 30 min. Reactions were terminated by the addition of ice-cold buffer A, and cell surface mAChR number was monitored by means of [3H]NMS binding. Values are reported as the specific binding of [3H]NMS (fmol/mg protein) and as the Oxo-M induced loss of cell surface [3H]NMS binding sites (percentage) relative to controls. Results from one of two experiments are shown.