Sensitivity of NR2B(W607) mutants to pH, glutamate, and glycine
| Subunit combination | pH IC50 | No. of oocytes | Glutamate EC50 | No. of oocytes | Glycine EC50 | No. of oocytes |
|---|---|---|---|---|---|---|
| μm | μm | |||||
| Wild-type NR1/NR2B | 7.3 ± 0.02 | 5 | 1.4 (1.3, 1.5) | 6 | 0.14 (0.12, 0.16) | 6 |
| NR1/NR2B(W607L)2-a | 7.5 ± 0.01 | 4 | 2.5 (2.4, 2.6) | 4 | 0.22 (0.21, 0.23) | 4 |
| NR1/NR2B(W607N) | 7.4 ± 0.01 | 3 | 2.7 (2.5, 3.0) | 4 | 0.25 (0.24, 0.26) | 4 |
| NR1/NR2B(W607A) | 7.3 ± 0.01 | 3 | 2.0 (1.8, 2.3) | 4 | 0.42 (0.35, 0.51) | 4 |
| NR1/NR2B(W607Y) | 7.3 ± 0.01 | 3 | 1.8 (1.7, 2.0) | 4 | 0.28 (0.25, 0.31) | 4 |
| NR1/NR2B(W607F) | 7.2 ± 0.01 | 3 | 1.6 (1.4, 1.8) | 4 | 0.12 (0.12, 0.13) | 4 |
IC50 values (mean ± standard error) for pH inhibition were determined by measuring responses to glutamate over a pH range of 6.5 to 8.5 as described previously (Kashiwagi et al., 1997). EC50 values for glutamate and glycine were determined from concentration-response curves for each agonist in the presence of 10 μm glycine (glutamate curves) or 10 μmglutamate (glycine curves). Values are the geometric mean (− standard error, + standard error).
↵2-a Data for glutamate and glycine at NR2B(W607L) are fromKashiwagi et al. (1997).