Temperature | Fast association constants (n = 3) | Slow association constants (n = 3) | ||
---|---|---|---|---|
kfast | t1/2 | kslow | t1/2 | |
min−1 | min | min−1 | min | |
4° | 4.6 ± 0.4 × 10−2 | 15 ± 1.5 | 1.8 ± 0.7 × 10−3 | 448 ± 177 |
20° | 3.7 ± 0.6 × 10−1 | 1.9 ± 0.3 | 9.1 ± 0.2 × 10−3 | 76 ± 2 |
37° | 4.8 ± 0.4 × 10−1 | 1.4 ± 0.1 | 2.2 ± 0.2 × 10−2 | 33 ± 5 |
Values for observed association rate constants kfastand kslow were obtained by nonlinear least-squares fitting to the data shown in Fig. 1a. t1/2 values were calculated from these kinetic constants.