Temperature | Dissociation constants (n = 3) | |
---|---|---|
koff | t1/2 | |
min−1 | min | |
4° | 1.7 ± 0.2 × 10−3 | 434 ± 56 |
20° | 9.1 ± 0.2 × 10−3 | 76 ± 2 |
37° | 4.3 ± 0.9 × 10−2 | 19 ± 5 |
Values of koff were obtained by nonlinear least-squares fitting to the data shown in Fig. 1b.t1/2 values were calculated from these kinetic constants.