Activation of nNOS in CHO hm1/nNOS cells by CCh or xanomeline
| Agonist | Antagonist | |||
|---|---|---|---|---|
| Atropine (30 nm) | Pirenzepine (150 nm) | |||
| CCh | Xanomeline | CCh | Xanomeline | |
| Emax 1-a | 7.44 ± 2.04 | 7.83 ± 2.01 | 6.85 ± 2.12 | 5.13 ± 1.91 |
| pEC50 (control) | 5.96 ± 0.11 | 8.34 ± 0.14 | 5.79 ± 0.07 | 7.79 ± 0.07 |
| pEC50(+antagonist) | 4.55 ± 0.13 | 7.10 ± 0.16 | 4.53 ± 0.09 | 6.66 ± 0.08 |
| n H 1-b | 1.79 ± 0.57 | 1.99 ± 1.18 | 1.47 ± 0.47 | 3.24 ± 1.89 |
The activity of nNOS was quantified by measuring the formation ofl-[3H]citrulline in response to agonist for 1 hr at 37° in HEPES buffer, as indicated in the text. Parameters derived from nonlinear regression analysis are presented as the mean ± standard error of four or five determinations conducted in triplicate. Emax and nH parameter values were constrained to be shared between each pair of agonist curves, in the absence or presence of antagonist (see the text). The basal parameter values were set to 1.