Table 1

Effect of test compounds on leptin secretion by differentiated 3T3-F422A adipocytes

Compound	Concentration	Leptin secretion
	μm	% of Control
CGP 52608	10	103 ± 8
CGP 55066	10	88 ± 3
CGP 55707	10	90 ± 4
Melatonin	10	114 ± 14
	1	128 ± 4
	0.1	132 ± 10
BRL 49653	0.2	19 ± 4
(racemate)	0.05	25 ± 12
	0.01	31 ± 5
	0.002	117 ± 16
BRL 49653	0.2	30 ± 14
R-(+)	0.05	25 ± 7
	0.01	48 ± 9
	0.002	92 ± 14
BRL 49653	0.2	13 ± 6
S-(−)	0.05	27 ± 14
	0.01	23 ± 4
	0.002	102 ± 11
15d-PGJ₂	10	61 ± 24
	1	85 ± 34
	0.1	110 ± 27
PGD₂	10	82 ± 28
	1	135 ± 27
	0.1	115 ± 18

Fully differentiated adipocytes were incubated for 72 hr with test compounds or vehicle, and cumulative leptin secretion was measured by radioimmunoassay. The leptin content in the supernatant of control cultures was 1.7 ± 0.22 ng/ml. Data are given as mean ± standard error for three determinations.