Compound | Concentration | [3H]E217βG uptake |
---|---|---|
μm | % control | |
None (control) | 100 | |
LTC4 | 10 | 22 ± 71-b |
α-Naphthyl-β-d-glucuronide | 1000 | 94 ± 1 |
p-Nitrophenyl-β-d-glucuronide | 1000 | 97 ± 5 |
Phenolphthalein glucuronide | 1000 | 5 ± 41-b |
MK571 | 5 | 33 ± 31-a |
CsA | 10 | 48 ± 11-a |
FL | 1000 | 94 ± 4 |
MTX | 1000 | 55 ± 41-a |
FL-MTX | 100 | 22 ± 71-b |
Sf9-Mrp2 membrane vesicles were incubated with 50 nm[3H]E217βG at 37° for 1 min with or without (control) the compounds as indicated. Net ATP-dependent transport was calculated by subtracting values in the presence of 4 mm5′-AMP from those in the presence of 4 mm ATP. Transport was expressed as percent of the control uptake. Data represent mean values of three to six determinations (± standard error).