Sensitivity of S. pombe mutants to γ-radiation
| Strain | ID50 (Krad, mean ± SD) |
|---|---|
| K rad | |
| wild-type | >120 |
| rad1 | 18.2 ± 2.7 |
| rad3 | 14.2 ± 0.7 |
| rad19 | 14.8 ± 1.2 |
| rad4 | 45.3 ± 12.0 |
| rad9 | 32.0 ± 10.0 |
| rad17 | 12.0 ± 2.0 |
| rad5 | 38.0 ± 7.0 |
| rad15 | >120 |
| rad8 | 49.0 ± 12.0 |
| rad10 | 28.5 ± 7.5 |
| rad16 | 14.5 ± 7.5 |
| rad20 | 42.5 ± 7.5 |
| rad13 | 71.0 ± 4.0 |
| rad2 | >120 |
| rad6 | 21.2 ± 2.7 |
| rad7 | >120 |
| rad11 | 50.6 ± 10.3 |
| rad12 | 52.5 ± 8.5 |
| rad18 | 12.7 ± 2.7 |
| rad14 | 73.0 ± 4.6 |
| rad21 | 17.5 ± 2.5 |
| rad22 | 41.0 ± 3.0 |
Sensitivity was evaluated by using a microtiter growth-inhibition assay after exposure to radiation. ID50 values are the average (± standard deviation) of at least three independent experiments. When exposed to a dose of γ radiation of 120 Krad wild-type, rad2, rad7, and rad15 exhibited 90%, 66%, 57%, and 64% cell growth, respectively.