Perfusion data for study 3
| Perfusion periods for each tubule | Present in bathing fluid | Number of tubules studied | Perfusion rate | Mean luminal Hg2+concentration | Content of Hg2+ in TCA-soluble tubule fraction | Content of Hg2+ in TCA-insoluble tubule fraction | Leak |
|---|---|---|---|---|---|---|---|
| nl/min | μm | fmol/mm | fmol/mm | nl/min | |||
| 1st 15 min | None | 6 | 6.42 ± 0.52 | 15.6 ± 0.58 | 0.21 ± 0.06 | ||
| 2nd 15 min | 200 μm DMPS | 6.31 ± 0.71 | 20.6 ± 0.403-a | 20.1 ± 9.32 | 74.9 ± 6.51 | 0.22 ± 0.06 | |
| 1st 15 min | None | 3 | 10.7 ± 1.23 | 17.8 ± 0.22 | 20.0 ± 4.74 | 396 ± 54.6 | 0.09 ± 0.01 |
| 2nd 15 min3-b | NA | NA | NA | NA | NA | NA | |
| 1st 15 min | None | 2 | 7.62 ± 0.10 | 17.5 ± 0.64 | 0.10 ± 0.00 | ||
| 2nd 15 min | None | 7.18 ± 0.19 | 17.0 ± 0.64 | 30.0 ± 8.45 | 271 ± 51.73-c | 0.11 ± 0.04 |
Values represent the mean ± standard error obtained from isolated perfused S2 segments of proximal tubules. Refer to Materials and Methods for details of the experimental design. In all cases [except as marked (3-b) below], 20 μmHg2+ and 80 μm GSH were present in the luminal fluid.
↵3-a Significantly different (p < 0.05) from the corresponding mean obtained during the first 15 min of perfusion.
↵3-b NA, not applicable, because tubules were not perfused for a second period of 15 min.
↵3-c Significantly different (p < 0.05) from the corresponding mean obtained from the tubules perfused with Hg2+ plus GSH in the lumen and exposed to DMPS in the bath during the same 15 min of perfusion.