The effect of AG treatment on LPS-stimulated decreases in total P450 content and P450 mRNAs and protein expression
| Time | LPS | LPS + AG | AG | |
|---|---|---|---|---|
| hr | ||||
| NO | 6 | 241.5 ± 23.63-150 | 112.0 ± 18.6 | 98.2 ± 24.6 |
| 24 | 122.8 ± 11.2 | 127 ± 23.2 | 108.7 ± 17.2 | |
| Cyp2c29 | 6 | 73.0 ± 13.4 | 61.9 ± 11.7 | 123.8 ± 18.9 |
| mRNA | 24 | 36.8 ± 8.73-150 | 52.4 ± 12.63-150 | 132.2 ± 22.4 |
| Cyp3a11 | 6 | 54.1 ± 6.33-150 | 67.2 ± 13.33-150 | 139.9 ± 34.9 |
| mRNA | 24 | 16.2 ± 5.4 | 25.7 ± 10.9 | 160.2 ± 43.0 |
| 2C-like protein | 24 | 64.4 ± 3.13-150 | 42.0 ± 11.93-150 | 98.9 ± 16.6 |
| 3A-like protein | 24 | 27.7 ± 7.43-150 | 18.1 ± 3.03-150 | 57.0 ± 10.93-150 |
| Total P450 | 6 | 75.8 ± 6.93-150 | 72.6 ± 6.73-150 | 78.9 ± 6.23-150 |
| 24 | 57.5 ± 5.23-150 | 46.2 ± 3.13-150 | 74.8 ± 8.93-150 |
Mice were injected with LPS followed by repeated injections of AG every 4 hr for 6 and 24 hr. Animals were killed at the indicated times after LPS injection; plasma, hepatic microsomal protein, and total RNA were isolated and analyzed. The data presented in the table represent the mean ± standard error of five mice per treatment group and are expressed as percentages of the saline-treated control group mean.
↵3-150 Significantly different from control, p < 0.05.