Gating parameters of wild type versus BTX-resistant channels and h0.5 values with and without local anesthetics.
| Gating parameters | μ1 wild-type | μ1-N434K | μ1-L437K |
|---|---|---|---|
| mV | |||
| Activation | |||
| E0.5 | −37.8 ± 2.7 | −35.0 ± 2.5 | −29.7 ± 2.7 |
| k a | 6.8 ± 0.9 | 8.7 ± 0.8 | 7.6 ± 0.6 |
| Inactivation (h∞) | |||
| h0.5(control) | −79.6 ± 0.9 | −72.3 ± 1.1 | −80.1 ± 1.2 |
| k h (control) | 7.1 ± 0.2 | 6.3 ± 0.2 | 7.4 ± 0.2 |
| h0.5 (benzocaine) | −112.6 ± 1.8 [−33]2-a | −94.4 ± 2.2 [−22.1]2-a | −98.3 ± 1.3 [−18.2]2-a |
| k h (benzocaine) | 9.3 ± 0.42-a | 7.1 ± 0.4 | 11.0 ± 0.52-a |
| h0.5 (lidocaine) | −77.1 ± 1.2 [2.5] | −69.9 ± 4.5 [2.4] | −80.6 ± 2.3 [−0.5] |
| k h (lidocaine) | 7.5 ± 0.3 | 6.6 ± 0.5 | 7.7 ± 0.4 |
| h0.5 (etidocaine) | −87.5 ± 2.1 [−7.9]2-a | −70.6 ± 1.7 [1.7] | −83.0 ± 3.8 [−2.9] |
| k h (etidocaine) | 10.1 ± 1.32-a | 6.0 ± 0.3 | 7.3 ± 0.3 |
The gating parameters were estimated as described in Wang and Wang (1998). The number of experiments per value for activation was 6–11 and for inactivation was 4–9. The differences in h0.5 with and without drug are listed in brackets.
↵2-a p < 0.05.