Table 1

K_m and K _i values for wild-type and the K70E, M184V, and K65R mutants of HIV-1 RT

Recombinant HIV-1 RT	K_m		K_i
Recombinant HIV-1 RT	dATP	dCTP	dTTP	PMEApp	3TCTP	AZTTP	ddCTP	d4TTP	ddATP
μm
Wild-type	0.49	0.61	0.80	0.07	1.16	0.08	0.14	0.03	0.29
K65R	0.34	0.57	0.60	0.36	1.33	0.25	0.26	0.1	0.75
				[5.1]	[<2]	[3.1]	[<2]	[3.2]	[2.6]
K70E	0.54	0.74	1.03	0.19	2.66	0.09	0.22	0.05	0.33
				[2.7]	[2.3]	[<2]	[<2]	[<2]	[<2]
M184V	0.61	0.70	0.81	0.08	14.35	0.06	0.24	0.03	0.42
				[<2]	[12.4]	[<2]	[<2]	[<2]	[<2]

K _m values are averages from 3–10 separate experiments. The average standard error for these K _mvalues is 13.2%. K _i values are averages from three to six separate experiments. The average standard error for these values is 16.0%. Numbers in brackets are the fold increases inK _i values from wild-type. Fold increases of < 2-fold are not statistically significant.