Mutant | Location | n | PGE1 | PGE2 | Iloprost |
---|---|---|---|---|---|
L84F | 2 TM | 2 | yes | yes | no |
L90F | 2 TM | 2 | yes | yes | no |
Q97S | 1 EX1-a | 1 | no | no | no |
P104R | 1 EX | 1 | no | no | no |
Y111A | 3 TM | 3 | yes | yes | no |
S120G | 3 TM | 4 | yes | yes | no |
ΔC182 | 2 EX | 1 | no | no | no |
R190N | 2 EX | 4 | yes | yes | no |
H226Y | 3 IN1-b | 3 | yes | yes | no |
Q299L | 7 TM | 3 | yes | yes | no |
L301F | 7 TM | 3 | yes | yes | no |
R302E | 7 TM | 3 | yes | yes | no |
R302Q | 7 TM | 3 | yes | yes | no |
L304Y | 7 TM | 7 | yes | yes | yes |
S305A | 7 TM | 3 | yes | yes | no |
F315Y | 7 TM | 1 | no | no | no |
CV-1 cells transiently transfected with mutant EP2 receptor cDNA and reporter cDNA were evaluated in the CRE-CAT assay. “Yes” signifies a functional response to agonist at concentrations <1 μm. Each mutant was tested in the indicated number of independent experiments (n), with each assay carried out in triplicate.