Table 1

Summary of Pharmacology of EP₂ single mutants

Mutant	Location	n	PGE₁	PGE₂	Iloprost
L84F	2 TM	2	yes	yes	no
L90F	2 TM	2	yes	yes	no
Q97S	1 EX^1-a	1	no	no	no
P104R	1 EX	1	no	no	no
Y111A	3 TM	3	yes	yes	no
S120G	3 TM	4	yes	yes	no
ΔC182	2 EX	1	no	no	no
R190N	2 EX	4	yes	yes	no
H226Y	3 IN^1-b	3	yes	yes	no
Q299L	7 TM	3	yes	yes	no
L301F	7 TM	3	yes	yes	no
R302E	7 TM	3	yes	yes	no
R302Q	7 TM	3	yes	yes	no
L304Y	7 TM	7	yes	yes	yes
S305A	7 TM	3	yes	yes	no
F315Y	7 TM	1	no	no	no

CV-1 cells transiently transfected with mutant EP₂ receptor cDNA and reporter cDNA were evaluated in the CRE-CAT assay. “Yes” signifies a functional response to agonist at concentrations <1 μm. Each mutant was tested in the indicated number of independent experiments (n), with each assay carried out in triplicate.

↵1-a extracellular loop.
↵1-b intracellular loop.