Table 1

Sites of oxaliplatin adducts in purified SV40 ORI region mapped by repetitive primer extension with ORI-U primer

Stop site (absolute position in SV40 sequence)	Position relative to stop site		Intensity
Stop site (absolute position in SV40 sequence)	5′	+3	Intensity	+2	+1	0	−1	−2	−3	3′
5066		`a`	`g`	`G`	`C`	`a`	`t`	`a`		S
5071		`g`	`a`	`G`	`A`	`a`	`a`	`g`		S
5072		`t`	`g`	`A`	`G`	`a`	`a`	`a`		I
5089		`g`	`g`	`G`	`G`	`a`	`a`	`t`		S
5090		`g`	`g`	`G`	`G`	`g`	`a`	`a`		S
5091		`t`	`g`	`G`	`G`	`g`	`g`	`a`		S
5100		`a`	`g`	`G`	`A`	`g`	`t`	`g`		S
5110		`a`	`g`	`G`	`T`	`c`	`t`	`t`		S
5111		`t`	`a`	`G`	`G`	`t`	`c`	`t`		I
5120		`t`	`g`	`G`	`A`	`c`	`c`	`t`		I
5138		`a`	`g`	`G`	`A`	`a`	`t`	`c`		S
5139		`g`	`a`	`G`	`G`	`a`	`a`	`t`		S
5141		`g`	`a`	`G`	`A`	`g`	`g`	`a`		I
5154		`a`	`a`	`G`	`T`	`t`	`t`	`t`		I
5159		`t`	`g`	`G`	`A`	`t`	`a`	`a`		I
5160		`a`	`t`	`G`	`G`	`a`	`t`	`a`		S
5161		`g`	`a`	`T`	`G`	`g`	`a`	`t`		I

SV40 DNA was treated with drugs for 4 hr and analyzed as described in the text and the legend to Fig. 2. The table is a composite of several independent experiments of stop sites induced by oxaliplatin in the SV40 ORI region. The sequences are for the bottom (template) strand of SV40 DNA arranged 5′ to 3′. Absolute position in the SV40 sequence (leftmost column) refers to the stop site residue (relative position 0). Although oxaliplatin adducts may be 1–2 bp from the stop site, relative position +1 is most likely to correspond to the adducted base. A qualitative determination of intensity of the individual stop sites; S, strong, I, intermediate, was made. Some weak sites (<∼1% intensity) are omitted from the table.