Table 1

Effects of L-DOPA and NGF on intracellular DCF label

% Labeled cells
no NGF+ NGF
No L-DOPA0.51  ± 0.370.48  ± 0.35
50 μm L-DOPA3.83  ± 0.661-150 0.51  ± 0.461-160
200 μm L-DOPA6.20  ± 0.821-150 0.96  ± 0.361-160

Five days after plating, cultures were exposed to 0, 50, or 200 μm L-DOPA with or without NGF (50 ng/ml) for 4 days. The cultures were then examined for cells labeled by DCF. Data are mean ± standard error (n = 6; at least 2000 cells counted per condition).

  • 1-150  p < 0.001 versus no L-DOPA.

  • 1-160  p < 0.001 for cultures treated with NGF/L-DOPA versus the same level of L-DOPA without NGF.