Amperometric spike characteristics
| No. of events | Interspike interval | SD/Mean | No. of molecules | imax | Width | t1/2 | |
|---|---|---|---|---|---|---|---|
| pA | msec | ||||||
| NGF (n = 9) | 23 ± 12 | 571.1 ± 94.1 | 1.70 ± 0.15 | 111,000 ± 27,000 | 7.1 ± 1.7 | 13.9 ± 1.6 | 3.5 ± 0.4 |
| NGF & L-DOPA (n = 8) | 55 ± 122-150 | 269.9 ± 68.42-150 | 1.39 ± 0.10 | 226,000 ± 55,0002-150 | 9.8 ± 1.5 | 22.5 ± 7.6 | 4.2 ± 0.5 |
Analysis of quantal release from neurite varicosities. Exposure of PC12 cells treated with NGF alone to 80 mm K+ evoked 214 release events from nine varicosities of a total of 16 recorded (see Fig. 3, inset), whereas stimulation of cells treated with NGF and 50 μm L-DOPA evoked 439 release events from eight varicosities of a total of 15 recorded. Data in the table are expressed as mean per cell ± standard error. Analysis of the entire population of events, rather than cell means, showed similar trends (quantal size and interspike intervals of the population are shown in Fig. 4).
↵2-150 p < 0.01 different from control group by Kolmogorov-Smirnov statistics.
imax, maximum peak amplitude.
t1/2, the width at half height.
SD Mean, standard deviation of the interspike interval divided by the mean interspike interval.