Table 4

Effects of apomorphine and NGF on GSH synthesis

GSHProteinGSH
μg/well μg/mg protein
Controls1.3  ± 0.04 (100%)615  ± 4.4 (100%)2.11  ± 0.02 (100%)
Apomorphine16.41  ± 0.84-150 (1262%)523  ± 2.0 (92%)31.37  ± 0.94-150 (1487%)
NGF2.19  ± 0.24-150 (169%)701  ± 7.04-160 (114%)3.12  ± 0.14-150 (148%)
Apomorphine & NGF18.1  ± 0.5 (1391%)547  ± 2.0 (89%)33.10  ± 1.20 (1569%)

Five days after plating, cultures were exposed to 50 μmL-DOPA, 10 μm apomorphine, or 50 ng/ml NGF for 24 hr. The cultures were then assayed for total GSH and protein levels. Data are mean ± standard error (n = 6).

  • 4-150p < 0.001 versus controls.

  • 4-160p < 0.01 versus controls.