Table 6

Effects of L-DOPA and BSO on GSH synthesis

GSHProteinGSH
μg/well μg/mg protein
Controls1.3  ± 0.04 (100%)615  ± 4.4 (100%)2.11  ± 0.02 (100%)
L-DOPA3.07  ± 0.26-150 (236%)609  ± 18 (99%)5.04  ± 0.16-150 (239%)
BSO0.58  ± 0.016-150 (45%)640  ± 8 (104%)0.91  ± 0.016-150 (43%)
L-DOPA & BSO0.78  ± 0.016-160 (60%)590  ± 17 (96%)1.32  ± 0.016-160 (62%)

Five days after plating, cultures were exposed to 50 μmL-DOPA or 10 μm BSO for 24 hr. The cultures were then assayed for total GSH and protein levels. Data are mean ± standard error (n = 6).

  • 6-150p < 0.001 versus controls.

  • 6-160p < 0.001 for cultures treated with L-DOPA versus L-DOPA & BSO.