Table 1

Steric aspects of diglyceride action

TreatmentDNA fragmentsBulk DNA breaksApoptosis
ng/106cells rad eq % control
Vehicle215  ± 21117  ± 192  ± 1
ara-C735  ± 931-a 1727  ± 1311-a 27  ± 71-a
1,2-sn-Dioctanoylglycerol + ara-C334  ± 691-b 736  ± 701-b 11  ± 31-b
2,3-sn-Dioctanoylglycerol + ara-C767  ± 1081-a 1765  ± 1971-a 28  ± 41-a
1,3-rac-Dioctanoylglycerol + ara-C720  ± 881-a 1828  ± 931-a 29  ± 41-a

HL-60 cells were exposed to ara-C (10 μm) for 6 hr in the absence or presence of sn-1,2-, sn-2,3-, and 1,3-rac-substituted isomers of dioctanoylglycerol (1,2-sn-diC8, 2,3-sn-diC8, and 1,3-rac-diC8) at equimolar concentrations (2.5 μm). Apoptotic DNA degradation was quantified by spectrofluorophotometry. Values reflex mean ± standard error of triplicate determinations.

    • 1-a Increased versus vehicle (p < 0.001).

    • 1-b Decreased versus ara-C (p < 0.001).