Table 3

Insensitivity of ara-CTP metabolism to diglyceride and sphingosine

Treatmentara-CTP formation
pmol/1.5 × 107 cells
Vehicle324  ± 31
1,2-sn-Dioctanoylglycerol304  ± 23
d-erythro-Sphingosine305  ± 27

HL-60 cells were exposed to ara-C (10 μm) for 6 hr in the absence or presence of synthetic preparations of diglyceride (2.5 μm) or sphingosine (850 nm). Intracellular levels of ara-CTP were then determined by high performance liquid chromatography as described in Experimental Procedures. Values reflect mean ± standard error of triplicate determinations.