DMBA metabolism by BMS2 cells and C3H10T½ cells
| Cells | TCDD1-a | DMBA metabolite1-b | Total | ||||
|---|---|---|---|---|---|---|---|
| Dihydrodiols | Phenols1-c,1-d | ||||||
| 5,6− | 8,9− | 10,11− | 3,4− | ||||
| pmol/106 cells/hr | |||||||
| BMS2 | − | 0 | 5 (24) | 2 (9) | 3 (14) | 11 (53) | 21 |
| + | 0 | 12 (28) | 4 (8) | 6 (13) | 22 (51) | 44 | |
| 10T1/2 | − | 0 | 1 (30) | 1 (19) | 1 (19) | 1 (32) | 4 |
| + | 0 | 5 (28) | 3 (18) | 3 (16) | 7 (38) | 18 | |
Numbers in parentheses are percent total metabolism.
↵1-a Cells were treated for 24 hours with 0.1% DMSO vehicle (control) or 10 nm TCDD.
↵1-b Values represent the mean of duplicate determinations from separate experiments.
↵1-c Total identifiable DMBA phenols, which include 2-, 3-, and 4-OH DMBA.
↵1-d 7- and 12-hydroxymethyl metabolites were not detectable above background.