Effect of Fe-NTA on oxygen consumption in E9 and MV5 cells
| Cells Addition | Oxygen uptake | ||
|---|---|---|---|
| Pyr/Mal | Succinate | Asc-TMPD | |
| nmol/min/5 × 106 cells | |||
| E9 | |||
| None | 18.9 ± 2.2 | 21.8 ± 1.6 | 41.0 ± 1.9 |
| Fe-NTA | 11.7 ± 2.22-a | 11.9 ± 2.32-a | 19.9 ± 2.52-a |
| Vitamin E | 19.2 ± 0.5 | 20.2 ± 0.8 | 40.7 ± 0.8 |
| Vitamin E and Fe-NTA | 18.9 ± 1.7 | 18.8 ± 2.7 | 36.6 ± 1.9 |
| MV5 | |||
| None | 21.0 ± 0.8 | 24.2 ± 0.8 | 47.9 ± 1.1 |
| Fe-NTA | 20.2 ± 0.8 | 24.8 ± 0.9 | 46.8 ± 1.0 |
| Vitamin E | 21.4 ± 2.5 | 23.9 ± 0.8 | 48.0 ± 0.5 |
| Vitamin E and Fe-NTA | 21.1 ± 0.2 | 22.1 ± 1.0 | 44.0 ± 0.6 |
After an overnight incubation with 0.1 mm BSO, E9 or MV5 cells (5 × 106 cells/ml) were incubated with or without 30 μm Fe-NTA and with or without 0.025 mm vitamin E for 12 hr. The cells were harvested and oxygen consumption was assayed as described in Materials and Methods and the legend to Fig. 5. Values represent the mean ± standard error of three experiments.
↵2-a p < 0.05 compared with the control E9 cells.