Effect of Fe-NTA on ATP levels in E9 and MV5 cells
| Cells Addition | ATP content | Effect of Fe-NTA | |
|---|---|---|---|
| −Fe-NTA | +Fe-NTA | ||
| pmol/mg protein | (%) | ||
| E9 | |||
| None | 9.4 ± 1.0 | 5.9 ± 0.8 | −373-a |
| 0.025 mmVitamin E | 10.5 ± 0.6 | 9.0 ± 1.3 | −14 |
| 0.05 mm Trolox | 9.8 ± 1.3 | 10.2 ± 1.3 | +4 |
| MV5 | |||
| None | 13.6 ± 0.7 | 11.0 ± 1.1 | −19 |
| 0.025 mm Vitamin E | 12.4 ± 0.2 | 12.0 ± 0.9 | −3 |
| 0.05 mm Trolox | 12.9 ± 0.6 | 11.5 ± 0.2 | −11 |
E9 or MV5 cells (0.5 × 106 cells/ml) were treated with 0.1 mm BSO for 15 hr and then were incubated with or without 30 μm Fe-NTA and the indicated additions for 12 hr. Levels of ATP were determined as described in Materials and Methods. Results represent the mean ± standard error of 4–12 experiments.
↵3-a p < 0.05.