Comparison of K app and IC50 values for crystal violet interaction with the AChR in the presence and absence of agonist
| Method | Kapp | |
|---|---|---|
| − Carb | + Carb | |
| nM | ||
| Fluorescence | 100 ± 46 (5) | 10 ± 3.4 (2) |
| [3H]Phencyclidine | 1200 ± 140 (2) | 50 ± 30 (6) |
| [3H]Acetylcholine | 860 ± 120 (3) | N.D. |
| 22Na flux | 1700 ± 840 (4) | N.D. |
K app and IC50 values were determined by the various methods as described in Experimental Procedures and the individual figure legends. The averages and S.D.s are shown with the number of individual experiments in parentheses. N.D. indicates the measurement was not done.