Requirements |
Molecules require two chelating macrocyclic rings for high activity |
Distance between metal-binding centers must be 9.5 to 11.5 Å |
Plane torsion of −60 to 30° and 120 to 140° are allowed |
Plane angles of 40 to 70° and 110 to 140° are allowed |
Maximize metal affinity for each macrocyclic ring |
Optimum ring size for cyclam rings: 14 atoms |
Features to avoid |
Plane torsion of 70 to 110° |
Plane angles 0 to 35° and 160 to 180° |
Adapted from Joao et al. (1995).