Conductance Level | I | II | III | IV | V | VI | VII | VIII |
---|---|---|---|---|---|---|---|---|
WT: − amplitude (pS) | 95 ± 0.7 | 86 ± 1.8 | 78 ± 0.6 | 66 ± 2.0 | 51 ± 1.3 | 36 ± 1.6 | 25 ± 0.7 | 17 ± 0.6 |
WT: − incidence (x/n) | 8/9 | 3/9 | 6/9 | 7/9 | 5/9 | 8/9 | 3/9 | 4/9 |
WT: − contribution (%) | 39 ± 10 | 34 ± 4.9 | 8.8 ± 4.4 | 23 ± 9.6 | 17 ± 5.4 | 15 ± 3.7 | 15 ± 1.2 | 7.3 ± 4.4 |
Q266H: − amplitude (pS) | 95 ± 0.9 | 86 ± 1.0 | 77 ± 1.7 | 66 ± 2.6 | 54 ± 1.2 | 37 ± 2.1 | 23 ± 0.5 | 14 |
Q266H: − incidence (x/n) | 7/7 | 2/7 | 5/7 | 4/7 | 3/7 | 4/7 | 2/7 | 1/7 |
Q266H: − contribution (%) | 33 ± 10 | 23 ± 18 | 24 ± 5.9 | 23 ± 5.2 | 17 ± 3.3 | 28 ± 10 | 12 ± 8.0 | 5 |
Amplitude and distribution of single-channel conductance states recorded in outside-out membrane patches, excised from HEK-293 cells expressing either α1-homomeric WT or Q266H GlyRs and held at −60 mV in response to low doses of glycine. Eight separate subconductance states were identified. Incidence refers to the number of patches (x) in which the various subconductance states were observed [total number of patches (n) was nine for WT and seven for Q266H GlyRs]. Contribution (%) refers to the mean and S.E.M. of the contribution of that particular conductance state to the total channel activity observed in each patch. The contribution (%) was calculated only from patches in which that particular conductance state was observed.